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目的 探讨黄芪提取物 -黄芪总皂甙对柯萨奇病毒B3(CVB3)感染的病毒性心肌炎(VMC)小鼠慢性期心肌肌浆网钙泵 (SERCA)活性变化的影响。方法 将BALB/C小鼠分为正常组(31只 )、模型组 (36只 )及黄芪组 (2 6只 ) ,模型组及黄芪组每只小鼠以 2× 10 4 TCID50 CVB3(TCID50 为10 -5 83)腹腔注射感染。黄芪组每鼠每天腹腔注射 0 15ml黄芪总皂甙注射液 ,正常及模型组予同体积的生理盐水。 3个月后 ,观察各组小鼠死亡率及心肌病理改变 ,检测血清心肌肌钙蛋白I(cTnI)含量及心肌组织中SERCA活性。结果 (1)和正常组、黄芪组相比 ,模型组小鼠死亡率显著增高 (P <0 0 5～ 0 0 0 1)。 (2 )感染 3个月后模型组、黄芪组心肌肌钙蛋白I(cTnI)及心肌病理改变与正常组无显著性差异 (P >0 0 5 )。 (3)模型组心肌SERCA活性显著低于正常组及黄芪组 (P <0 0 1)。结论 黄芪总皂甙可以降低VMC小鼠的死亡率 ,对慢性期病毒感染所致心肌组织SERCA功能下调有逆转作用
Objective To investigate the effects of astragalus extract, total saponin of astragalus membranaceus, on the activity of sarcoplasmic reticulum calcium pump (SERCA) in myocardium of mice infected with Coxsackie virus B3 (CVB3) infected with viral myocarditis (VMC). Methods BALB/C mice were divided into three groups: normal group (31), model group (36) and astragalus group (26). The model group and Huangqi group had 2 × 10 4 TCID50 CVB3 (TCID50 was 10 -5 83) Intraperitoneal injection. The rats in the Astragalus group were injected intraperitoneally with 0 15 ml of total astragalus injection per mouse, normal and model groups were given the same volume of normal saline. After 3 months, the mortality and myocardial pathological changes of the mice were observed. Serum cTnI levels and SERCA activity in myocardial tissue were measured. Results (1) Compared with the normal group and the Astragalus group, the mortality rate of mice in the model group was significantly higher (P <0 0 5 ~ 0 0 0 1). (2) After 3 months of infection, the cardiac troponin I (cTnI) and myocardial pathological changes in the model group and the Huangqi group were not significantly different from those in the normal group (P > 0 05). (3) The SERCA activity in the model group was significantly lower than that in the normal group and the Huangqi group (P < 0 01). Conclusion Astragalus saponins can reduce the mortality of VMC mice and reverse the down-regulation of SERCA function in myocardium caused by chronic viral infection.