通过药物二乙基亚硝胺（ＤＥＮ）诱发大鼠肝癌，取肝癌白色结节用胶原酶消化，体外培养建立肝癌细胞系，筛选出对约氏疟原虫子孢子敏感的大鼠肝癌细胞系，建立了大鼠肝癌细胞──约氏疟原虫体外培养系统。约氏疟原虫子孢子接种单层培养的肝癌细胞，４８ｈ可见红外期裂殖体，７２ｈ后将培养细胞胰酶消化，消化下的细胞及培养上清一并离心，悬液接种健康小鼠，可使之感染疟疾。 Rat liver cancer was induced by diethylnitrosamine (DEN), and the white nodules of hepatocarcinoma were digested with collagenase and cultured in vitro to establish a hepatocellular carcinoma cell line. The rat hepatocarcinoma cell line that was sensitive to the sporozoites of Plasmodium yoelii was screened out. Established rat hepatocarcinoma cells - P. yoelii in vitro culture system. Plasmodium yoelii sporozoites were inoculated with monolayer culture of hepatoma cells, infiltrative sporozoites can be seen 48h after 72h cultured cells were trypsinized, digested cells and culture supernatant centrifuged, the suspension inoculated healthy mice, Can make it infected with malaria.