论文部分内容阅读
目的 分析肾综合征出血热山东分离株鲁宁 84刘株的分子特征。方法 应用逆转录聚合酶链反应 (RT PCR)扩增鲁宁 84刘株M和S片段全基因 ,克隆于T载体 ,纯化后测定序列 ,应用DNASTAR软件比较分析。结果 鲁宁 84刘株 (JNL株 )的M片段的全基因序列共 36 15个核苷酸 ,编码 1135个氨基酸 ,S片段的全基因序列共 16 98个核苷酸 ,编码 4 2 9个氨基酸 ,为HTN型毒株。JNL株与HTN型毒株M和S全基因序列差异分别高达 2 0 0 %～ 2 0 6 %和 15 5 %～ 16 0 % ,基于核苷酸序列的种系发生分析结果也可以看到 ,鲁宁 84刘株不同于国内外其他的分离株 ,处于独立的分支。结论 山东地区鲁宁 84刘株流行株是与国内外HTN型毒株不同的新的HTN亚型病毒。
OBJECTIVE: To analyze the molecular characteristics of Shandong isolate Lu-ning 84 Liu hemorrhagic fever with renal syndrome. Methods The full length M and S fragments of Lüin 84 Liu strain were amplified by reverse transcription polymerase chain reaction (RT PCR) and cloned into T vector. After purification, the sequences were sequenced and analyzed by DNASTAR software. Results The full-length M gene fragment of Lnning 84 strain (JNL strain) was 3615 nucleotides in length, encoding 1135 amino acids. The full-length sequence of the S fragment was 1698 nucleotides in length, encoding 429 amino acids , HTN strain. The sequence differences of M and S genes between JNL strain and HTN strain were as high as 200% to 206% and 15% to 160%, respectively. Based on the phylogenetic analysis of nucleotide sequence, Luling 84 Liu strains different from other isolates at home and abroad, in an independent branch. Conclusion The strain of Lonin 84 Liu strain in Shandong province is a new type of HTN subtype that differs from HTN strains at home and abroad.