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采用沙培试验对NaCl胁迫条件下野生及栽培大豆叶片中可溶性糖、脯氨酸、可溶性蛋白质含量以及地上部及根部Na+、K+含量进行了测定。结果表明:0～200 mmol.L-1浓度范围内,随着NaCl胁迫浓度的增加,野生大豆叶片可溶性糖、脯氨酸含量均逐渐增加,可溶性蛋白含量先升高后降低;栽培大豆脯氨酸含量逐渐升高,可溶性糖及蛋白含量均先升高后降低;2种大豆地上部及根系Na+含量均随胁迫程度的增加而增加;K+含量均随胁迫程度增加下降。在NaCl胁迫下,2种大豆均可通过渗透调节来抵抗盐的胁迫,野生大豆的渗透调节能力强于栽培大豆。
The contents of soluble sugar, proline, soluble protein and the contents of Na +, K + in shoots and roots of wild and cultivated soybean leaves under NaCl stress were determined by sand culture test. The results showed that the content of soluble sugar and proline in wild soybean leaves increased gradually with the increase of NaCl stress concentration at 0-200 mmol·L-1, while the content of soluble protein increased at first and then decreased. The content of soluble sugar and protein both increased at first and then decreased. The content of Na + in shoots and roots of two kinds of soybean increased with the increase of stress degree. K + content decreased with the degree of stress increased. Under NaCl stress, osmotic adjustment was used to resist salt stress in both of the two soybean species. The osmotic adjustment ability of wild soybean was stronger than that of cultivated soybean.