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目的分析湘潭市2014年-2015年禽流感外环境监测结果,了解湘潭市外环境中禽流感病毒流行特点和趋势,为禽流感防控提供参考依据。方法采集湘潭市禽流感外环境监测点标本411份,用实时荧光定量PCR进行甲型流感病毒(Flu A)核酸检测,Flu A阳性标本进行H5、H7、H9分型。结果 411份禽流感外环境标本中,Flu A阳性160份,占38.93%;H5亚型49份,阳性率为11.92%;H7亚型19份,阳性率为4.62%;H9亚型124份,阳性率为30.17%;其他型25份,占6.08%;2014年-2015年禽流感病毒阳性高峰期在冬春季节;不同类别标本以清洗禽类污水阳性率最高,不同监测场所以禽类交易市场阳性率最高。结论禽流感外环境中存在致病性禽流感潜在传播风险;加强禽流感外环境监测,加强禽类从业人群的健康教育,对于防控人禽流感具有重要意义。
Objective To analyze the external environment monitoring results of bird flu in Xiangtan City from 2014 to 2015, understand the epidemic characteristics and trends of avian influenza virus in the external environment of Xiangtan City, and provide reference for the prevention and control of avian influenza. Methods 411 samples were collected from external environment monitoring points of bird flu in Xiangtan City. Flu A nucleic acid was detected by real-time fluorescence quantitative PCR, and H5, H7 and H9 were typed in Flu A positive samples. Results Among 411 samples of peripheral blood samples, Flu A positive was 160, accounting for 38.93%; H5 subtype 49, the positive rate was 11.92%; H7 subtype was 19, the positive rate was 4.62%; H9 subtype 124, The positive rate was 30.17%; the other 25 types, accounting for 6.08%; the highest positive peak of avian influenza virus was in winter and spring in 2014-2015; the positive rate of different categories of samples was the highest in washing poultry wastewater; in different monitoring sites, the poultry market was positive The highest rate. Conclusion There is a potential risk of transmission of pathogenic avian influenza in the external environment of bird flu. Strengthening environmental monitoring of bird flu and strengthening health education of poultry workers are of great significance for the prevention and control of bird flu.