论文部分内容阅读
体外研究PEEK/HA生物复合材料对3T3成纤维细胞的影响,评价该材料的生物相容性。制备不同HA含量的PEEK/HA和PEEK/SPEEK/HA生物复合材料,与3T3成纤维细胞共培养,倒置相差显微镜观察该材料对细胞形态影响,MTT法研究该材料对细胞增殖作用,评价细胞毒性,流式细胞术研究该材料对细胞凋亡的影响,扫描电镜观察该细胞在材料表面黏附形态。细胞呈长梭形贴壁生长,胞体透明,大部分呈梭形和多角形,生长态势良好。随着浸提液浓度增大,材料对细胞增殖作用显著下降,PEEK/HA复合材料随着HA含量增加,细胞增殖作用逐渐增强,PEEK/SPEEK/HA复合材料与PEEK/HA相比,对细胞增殖作用较强,细胞毒性级别为Ι级,说明该材料对3T3细胞无毒性。细胞凋亡率随PEEK/SPEEK/HA材料中的HA含量升高而降低。SPEEK的加入使PEEK/HA复合材料后黏附率增大,随PEEK/SPEEK/HA中HA含量增加,细胞黏附率增加,SPEEK和HA可改善材料的黏附性能。SEM观察发现3T3细胞能够在PEEK/SPEEK/HA复合材料表面黏附、伸展和生长。PEEK/SPEEK/HA复合材料生物相容性良好,为该人工骨替代材料的临床前研究提供实验数据和科学依据。
The effect of PEEK / HA biocomposite on 3T3 fibroblasts was evaluated in vitro to evaluate the biocompatibility of this material. The PEEK / HA and PEEK / SPEEK / HA composites with different content of HA were prepared and co-cultured with 3T3 fibroblasts. The morphological changes of the cells were observed by inverted phase contrast microscope. The cell proliferation was evaluated by MTT assay and the cytotoxicity Flow cytometry was used to study the effect of the material on apoptosis. The adhesion morphology of the material on the surface of the material was observed by scanning electron microscopy. Cells were long fusiform adherent growth, cell body transparent, mostly fusiform and polygonal, good growth. Compared with PEEK / HA, PEEK / SPEEK / HA composites could significantly inhibit the cell proliferation when PEEK / HA / PEEK / HA composites increased with the increase of HA concentration, Strong proliferation, cytotoxicity level Ι, indicating that the material on the 3T3 cells non-toxic. The apoptosis rate decreased with the increase of HA content in PEEK / SPEEK / HA material. After the addition of SPEEK, the adhesion rate of PEEK / HA composites increased. With the increase of HA content in PEEK / SPEEK / HA, the cell adhesion rate increased. SPEEK and HA could improve the adhesion of the materials. SEM observation showed that 3T3 cells could adhere, spread and grow on the surface of PEEK / SPEEK / HA composites. The biocompatibility of PEEK / SPEEK / HA composites is good, which provides experimental data and scientific evidences for preclinical studies of alternative materials of artificial bone.