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目的观察人宫颈癌癌基因(HCCR)mRNA在肝癌和癌旁正常组织以及肝癌患者和正常人外周血单个核细胞(PBMC)中的表达水平。方法TRIZOL法提取肝癌组织和癌旁正常肝组织以及肝癌患者和正常人的PBMC的RNA,用RT-PCR技术分析HCCR基因在不同样品中的mRNA表达水平。结果在肝癌组织中,HCCR的mRNA相对表达强度为0.776±0.101,癌旁正常组织中的相对表达强度0.470±0.154,两者相比,P<0.05。在PBMC中,肝癌患者HCCR mRNA的表达强度为0.256±0.069,而正常人未检测出,两者相比,P<0.05。结论HCCR基因可能参与了肝癌的发生与发展,与肝癌的病理过程有一定的相关性。
Objective To observe the expression of human cervical cancer oncogene (HCCR) mRNA in peripheral blood mononuclear cells (PBMCs) of hepatocellular carcinoma and adjacent normal tissues, as well as in patients with hepatocellular carcinoma and normal human. Methods TRIZOL method was used to extract RNA from PBMC of HCC tissue, adjacent normal liver tissue and HCC patients and normal individuals. The mRNA expression of HCCR gene in different samples was analyzed by RT-PCR. Results The relative expression intensity of HCCR mRNA was 0.776 ± 0.101 in hepatocellular carcinoma, and 0.470 ± 0.154 in normal tissues. The difference was significant (P <0.05). In PBMC, the expression of HCCR mRNA in HCC patients was 0.256 ± 0.069, but not in normal subjects, P <0.05. Conclusions HCCR gene may be involved in the occurrence and development of hepatocellular carcinoma and has some correlation with the pathological process of hepatocellular carcinoma.