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本研究为了解南京地区儿童腺病毒(ADV)感染的流行特点及型别,收集2010年8月至2011年7月南京医科大学附属南京儿童医院住院及门诊呼吸道感染患儿的鼻咽抽吸物(NPA)及咽拭子(NPS)共644例,采用巢氏聚合酶链反应法(Nested-PCR)检测ADV hexon基因,将阳性PCR扩增产物进行测序、同源性和进化分析。同时对12种其他呼吸道相关病毒进行PCR检测,包括人博卡病毒(HBoV),呼吸道合胞病毒(RSV),人鼻病毒(HRV),副流感病毒1～4型(PIV1-4),流感病毒A和B(IFVA/B),人偏肺病毒(HMPV),冠状病毒NL63和HKU1(HCoV-NL63和HCoV-HKU1)。结果显示:644例标本中共检出ADV阳性扩增产物171份,检出率为26.55%,3型120例(70.18%,120/171),7型16例(9.36%,16/171),1型12例(7.02%,12/171),2型10例(5.85%,10/171),5型6例(3.51%,6/171),6型3例(1.75%,3/171),57型3例(1.75%,3/171),41型1例(0.58%,1/171)。ADV感染呈全年散发,其发病高峰主要在4～7月。以7岁以下儿童多见(96.49%)。171例ADV感染患儿中有99例(57.89%)存在混合感染,其中以呼吸道合胞病毒(RSV)、人鼻病毒(HRV)多见。ADV阳性患儿诊断以下呼吸道感染为主(63.16%),肺炎占30.41%。结论:ADV是2010年8月到2011年7月南京地区儿童呼吸道感染的重要病原之一,其优势流行株为3型,长期监测其流行型别具有重要意义。
In order to understand the epidemiological features and types of adenovirus infection in children in Nanjing, this study collected nasopharyngeal aspirates from hospitalized and outpatient respiratory patients in Nanjing Children’s Hospital from August 2010 to July 2011. (NPA) and throat swab (NPS). A total of 644 cases of ADV hexon gene were detected by nested PCR. The positive PCR products were sequenced, homology and phylogenetic analysis. Meanwhile, 12 kinds of other respiratory related viruses were detected by PCR, including human Bocavirus (HBoV), Respiratory syncytial virus (RSV), human rhinovirus (HRV), parainfluenza virus type 1 and 4 (PIV1-4), influenza Viruses A and B (IFVA / B), human metapneumovirus (HMPV), coronaviruses NL63 and HKU1 (HCoV-NL63 and HCoV-HKU1). The results showed that 171 positive amplification products were found in 644 specimens, with a detection rate of 26.55%, type 3 120 (70.18%, 120/171), type 7 16 (9.36%, 16/171) 6 cases (3.51%, 6/171) in type 1, 12 cases in type 1 (7.02%, 12/171), 10 cases in type 2 ), 57 cases in 3 cases (1.75%, 3/171) and 41 cases in 1 case (0.58%, 1/171). ADV infection was distributed throughout the year, the peak incidence in 4 to 7 months. Most common in children under 7 years of age (96.49%). In 171 children with ADV infection, 99 cases (57.89%) had mixed infection, of which respiratory syncytial virus (RSV) and human rhinovirus (HRV) were more common. ADV-positive children diagnosed the following respiratory infections (63.16%), pneumonia accounted for 30.41%. Conclusion: ADV is one of the most important pathogens of respiratory tract infections in children in Nanjing from August 2010 to July 2011. The predominant epidemic strain is type 3, and it is of great significance to monitor its prevalence in long term.