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本文研究青梅核醇提物乙酸乙酯相(PMS-E)和青梅花醇提物正丁醇相(PF-B)对小鼠高尿酸血症的拮抗效应。采用酵母膏灌胃建立小鼠高尿酸血症模型,碱性磷钨酸盐法测定小鼠血尿酸水平,试剂盒法测定小鼠血清肌酐、尿素氮和白球比水平以及血清和肝脏中黄嘌呤氧化酶(XO)活性。结果表明,PMS-E和PF-B均能显著降低高尿酸血症小鼠血尿酸水平(p<0.05)和白球比,并能显著升高血清肌酐(p<0.05)和尿素氮水平(p<0.05);并且在一定程度上促进动物的体重增长,而对肝体指数和肾体指数均无显著影响。此外,PMS-E和PF-B可有效抑制血清和肝脏XO活性。本研究结果为用PMS-E和PF-B开发降尿酸、抗痛风的膳食补充剂奠定了基础。
In this paper, the antagonistic effect of PMS-E and PF-B on the hyperuricemia in mice was studied. The mice model of hyperuricemia was established by intragastric administration of yeast extract, the level of serum uric acid was measured by alkaline phosphotungstate method, the levels of serum creatinine, urea nitrogen and white ball in mice were determined by kit method, and the levels of xanthine Oxidase (XO) activity. The results showed that both PMS-E and PF-B could significantly reduce serum uric acid level (p <0.05) and white ball ratio in hyperuricemia mice and significantly increase serum creatinine (p <0.05) and urea nitrogen level <0.05), and to a certain extent, promote the growth of animals without significant effect on liver index and kidney index. In addition, PMS-E and PF-B are effective in inhibiting serum and liver XO activity. The results of this study lay the foundation for the development of uric acid-and anti-gout dietary supplements using PMS-E and PF-B.