采用高效液相色谱法（ＨＰＬＣ）对顺铂注射液中顺铂的含量进行了测定。以μ－ＢｏｎｄａｐａｋＮＨ＿２为固定相，８５％乙腈－水溶液为流动相，以腺嘌呤作内标，在流速为１．５ｍｌ／ｍｉｎ时，顺铂和内标的保留时间分别为３．１５，４．４６ｍｉｎ、检出波长为２１０ｎｍ、最低检测限为３ｎｇ。标准曲线的线性范围为１．２１～４２．３５μｇ／ｍｌ（ｒ＝０．９９９３），方法回收率为１００．３％，相对标准偏差＜４．２７３％。 The content of cisplatin in cisplatin injection was determined by high performance liquid chromatography (HPLC). Using μ-BondapakNH_2 as the stationary phase, 85% acetonitrile-water as the mobile phase and adenine as the internal standard, the retention times of cisplatin and internal standard were respectively 3.15 and 4.46min at the flow rate of 1.5ml / min , Detection wavelength of 210nm, the minimum detection limit of 3ng. The linear range of the standard curve was 1.21-42.35μg / ml (r = 0.9993). The recovery rate was 100.3% and the relative standard deviation was 4.273%.