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目的 :观察维甲酸受体 β(RetinoicAcidReceptorbeta ,RARβ)基因联合全反式维甲酸 (All TransRetinoicAcid ,ATRA)对荷口腔鳞癌裸鼠体内抗肿瘤的作用和安全性。方法 :pSG5RARβ质粒转化、扩增、酶切鉴定。将 1× 10 7/ml密度的Tca8113细胞悬液 0 .2ml,分别接种于 2 0只裸鼠双侧臀部皮下。 2周后 ,随机将其分为对照组 ( 6只 )、ATRA治疗组 ( 8只 )、RARβ +ATRA治疗组 ( 6只 )。于接种后第 2、3周末 2次瘤内注射DNA 脂质体复合物 ,每只注射总量 6.75 μg。第 2周末开始 ,ATRA组和转RARβ基因组管饲给予 15mg/kg剂量的ATRA ,1次 /d ,5d/周× 4周。每 4d观察记录肿瘤最大径 (a)和最大垂直相交径 (b) ,观察记录动物一般情况。给药 4周后处死动物 ,切取肿瘤、称重。结果 :治疗结束时 ,对照组、ATRA、RARβ +ATRA治疗组肿瘤平均体积分别为 3 111.2mm3 ,14 2 8.8mm3 ,65 3 .9mm3 。治疗后 ,各组肿瘤平均体积分别增加 75 .1倍、66.1倍和 2 1.9倍 ,RARβ +ATRA治疗组与对照组差异有显著性 (P =0 .0 479)。 3组裸鼠平均瘤重分别为 2 .79g、0 .91g和 0 .5 7g ,ATRA和RARβ +ATRA治疗组与对照组相比差异有统计学意义 (P <0 .0 5 )。ATRA治疗组抑瘤率为 67.4% ,RARβ +ATRA治疗组抑瘤率为 79.6%。 3组动物平均体重无
Objective: To investigate the antitumor effect and safety of Retinoic Acid Receptor β (RARβ) gene combined with All Trans retinoic Acid (ATRA) on oral squamous cell carcinoma in nude mice. Methods: Plasmid pSG5RARβ was transformed, amplified and identified by restriction enzyme digestion. 0.2 ml Tca8113 cell suspension of 1 × 10 7 / ml density was inoculated into 20 nude mice subcutaneously on both buttocks. Two weeks later, they were randomly divided into control group (n = 6), ATRA treatment group (n = 8) and RARβ + ATRA treatment group (n = 6). Two intratumoral injections of DNA liposome complexes were given at the second and third weekend after inoculation with a total of 6.75 μg per injection. Beginning at the second weekend, ATRA and RARβ genomes were given a 15 mg / kg dose of ATRA by gavage once a day for 5 days for 4 weeks. The maximum diameter of tumor (a) and the maximum vertical intersection (b) were recorded every 4 days. The general situation of animals was observed and recorded. Animals were sacrificed 4 weeks after the administration, tumors were removed and weighed. Results: At the end of treatment, the mean tumor volumes of the control group, ATRA and RARβ + ATRA treatment groups were 3 111.2 mm 3, 14 2 8.8 mm 3 and 65 3 .9 mm 3, respectively. After treatment, the mean volume of tumor in each group increased by 75.1 times, 66.1 times and 2.9 times, respectively. The difference between the RARβ + ATRA treatment group and the control group was significant (P = 0.047). The average tumor weight of the three groups of nude mice were 2.79g, 0.91g and 0.57g, respectively. There was significant difference between the ATRA and RARβ + ATRA groups and the control group (P <0.05). The inhibition rate of ATRA treatment group was 67.4%, and the inhibition rate of RARβ + ATRA treatment group was 79.6%. The average weight of 3 groups of animals had no