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目的:观察熟地黄中有效成分5-羟甲基糠醛(5-HMF)对高浓度皮质酮(CORT)致海马神经元损伤及学习记忆相关蛋白学习记忆相关蛋白(GCR)、脑源性神经营养因子(BDNF)、血清和糖皮质激素调节蛋白激酶(SGK)蛋白表达的影响。方法:用24h新生大鼠原代培养海马神经细胞。第8天细胞用药处理,将细胞分为3组:正常对照组、模型组及5-HMF组。24h后,通过MTT法测定细胞活性,生化方法检测衰老特异性指标β-半乳糖苷酶活性,Western Blot检测学习记忆相关蛋白GCR、BDNF、SGK的基因表达。结果:与正常组相比,模型组细胞活力显著下降,β-半乳糖苷酶活性显著升高,GCR、BDNF、SGK蛋白表达显著性降低;0.5mg/L的5-HMF明显提高细胞活力,降低β-半乳糖苷酶活性,提高模型细胞GCR、BDNF、SGK基因表达(P<0.05,P<0.01)。结论:0.5mg/L 5-HMF可以保护大鼠海马神经细胞免遭高浓度皮质酮的损伤,通过调节GCR、BDNF、SGK的基因表达,可能在延缓学习记忆功能退化中发挥作用。
OBJECTIVE: To observe the effect of 5-hydroxymethylfurfural (5-HMF), an effective component of Rehmannia glutinosa, on hippocampal neuronal damage induced by high-concentration corticosterone (CORT) and learning and memory-related proteins (GCR) and brain-derived neurotrophic nutrition Effects of factors (BDNF), serum and glucocorticoid-regulated protein kinase (SGK) protein expression. METHODS: Primary hippocampal neurons were cultured in 24 h newborn rats. On the 8th day, the cells were treated with drugs and the cells were divided into 3 groups: normal control group, model group, and 5-HMF group. After 24 hours, cell viability was measured by MTT assay, specific activity of β-galactosidase was detected by biochemical methods, and gene expressions of learning and memory-related proteins GCR, BDNF, and SGK were detected by Western Blot. RESULTS: Compared with the normal group, the cell viability of the model group was significantly decreased, the activity of β-galactosidase was significantly increased, and the expression of GCR, BDNF, and SGK proteins was significantly decreased; 0.5-mg/L 5-HMF significantly increased cell viability. The β-galactosidase activity was decreased and the expression of GCR, BDNF and SGK genes in model cells was increased (P<0.05, P<0.01). Conclusion: 0.5mg/L 5-HMF can protect rat hippocampal neurons from high concentrations of corticosterone, and it may play a role in delaying the decline of learning and memory function by regulating gene expression of GCR, BDNF and SGK.