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目的:制作梗死区集中在小鼠大脑胡须体觉皮质的局灶性脑缺血模型。方法:小鼠麻醉后,经右侧顶叶皮质处开颅,显微镜和内在光信号成像下结扎大脑中动脉分支2～3支。应用激光多普勒血流仪检测结扎前、后局部脑血流情况,TTC染色检测梗死面积大小,TUNEL染色标记细胞死亡情况,细胞色素氧化酶染色确定体觉皮质和梗死部位的关系。结果:大脑中动脉分支结扎后,梗死中心及周围局部脑血流明显下降。缺血后3 d,TTC染色示梗死区位于胡须体觉皮质,梗死面积约占脑冠状切面面积的15%,TUNEL染色示死亡细胞位于胡须体觉皮质,不累及白质,细胞色素氧化酶染色示胡须体觉皮质结构破坏。结论:可选择性将小鼠具有单一功能的胡须体觉皮质制作成局灶性脑缺血模型,为脑缺血研究的动物模型提供更多选择。
OBJECTIVE: To establish a focal cerebral ischemia model in which the infarct zone concentrates on the bearded somatic cortex of mouse brain. Methods: After the mice were anesthetized, the cranial cortex of the right parietal cortex was opened and the branches of the middle cerebral artery were ligated 2 to 3 under microscope and internal light signal. The cerebral blood flow before and after ligation was detected by laser Doppler flowmetry. The area of infarction was detected by TTC staining and the cell death was marked by TUNEL staining. The relationship between somatosensory cortex and infarction was determined by cytochrome oxidase staining. Results: After the branches of the middle cerebral artery were ligated, the cerebral blood flow in the center of the infarction and the surrounding area decreased obviously. At 3 days after ischemia, TTC staining showed that the infarct area was located in bearded body cortex. The infarction area accounted for 15% of the coronal section area. The TUNEL staining showed that the dead cells were located in bearded body cortex and did not affect the white matter. Cytochrome oxidase staining Beards body cortical structure destroyed. CONCLUSIONS: The bearded somatosensory cortex, with a single function in mice, can be selectively made into a focal cerebral ischemia model, providing more choices for animal models of cerebral ischemia.