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外周血造血干细胞移植联合大剂量化疗已成为目前某些恶性肿瘤根治性治疗手段，而干细胞体外冷冻技术为其关键之一。目前国内外有关超低温冷冻对造血干细胞及不同淋巴结亚群存活的影响是否相同的报道少见。本研究采用程控降温系统将从外周血采集的单个核细胞(PBMC）逐步降温至－
Peripheral blood stem cell transplantation combined with high-dose chemotherapy has become a radical treatment of some malignant tumors, and stem cell freezing technology in vitro is one of the key. At present, there are few reports about the influence of cryogenic freezing on the survival of hematopoietic stem cells and different lymph node subgroups at home and abroad. In this study, a programmed cooling system was used to gradually cool mononuclear cells (PBMCs) collected from peripheral blood to -