论文部分内容阅读
以泥蚶为母本、毛蚶为父本,运用同步催产法,同时收集泥蚶的卵和毛蚶的精子进行人工杂交,采用普通光镜和荧光显微镜,对泥蚶(♀)×毛蚶(♂)受精及胚胎发育的细胞学过程进行了连续观察。结果显示,毛蚶的精子可以附着并穿过泥蚶的卵膜进行受精,并激活卵子减数分裂使其释放第一极体(PB1)和第二极体(PB2),能够形成雌、雄原核并发生原核联合,接着受精卵开始进行卵裂;杂交受精率统计为60.2%,发育速度较自交组慢;杂交胚胎发育前两次卵裂发育基本正常,后期才出现明显畸形;囊胚期持续时间很长,只能发育到担轮幼虫期,且纤毛越来越长,最后全部死亡。另外,在实验中发现有多精入卵和多极分离现象。
The experiment was carried out by using the method of synchronous oxytocin and collecting the sperm of the eggs and the furrows of the mudgrass with the muddy mound as the female parent and the furrow as the male parent, using ordinary light microscope and fluorescence microscope, The cytology process of fertilization and embryo development was continuously observed. The results showed that the sperm of Rana spp. Could attach to and pass through the oyster of the sod, and activate the oocyte meiosis to release the first polar body (PB1) and the second polar body (PB2), forming female and male pronuclei And the prokaryotic union occurred. Then the fertilized eggs began to undergo cleavage. The fertilization rate of the hybrid was 60.2%, which was slower than that of the selfed group. The development of the two cleavage embryos before and after hybrid embryo development was normal and later showed obvious deformity. It lasts for a long time and can only develop to the larval stage of the larvae. The cilia are getting longer and longer and eventually all die. In addition, in the experiment found more eggs and multipolarisation phenomenon.