论文部分内容阅读
免疫细胞化学和电镜观察人乳头瘤病毒（ＨＰＶ）形态发生结果表明：该病毒抗原见之于棘层、颗粒层和角质层细胞核内；毒粒则仅见之于颗粒层及角质层。从而提示了棘层以上的细胞层次为ＨＰＶ复制、装配和发育成熟的主要场所。本研究还观察到了ＨＰＶ装配的过渡阶段，宿主细胞的“溶解”及含有ＨＰＶ颗粒的细胞不一定都出现空泡等现象，从而提示了该病毒可因细胞溶解而释放，并造成上皮内扩散和再感染；空泡细胞不能作为诊断该病毒感染的可靠依据。
Immunocytochemistry and electron microscopy Morphology of human papillomavirus (HPV) showed that the virus antigen was found in the stratum spinosum, granular layer and stratum corneum nucleus. The virulence of the virion was found only in granular layer and stratum corneum. Suggesting that the cell level above the spinous layer is the major site for HPV replication, assembly and developmental maturation. This study also observed the HPV assembly of the transitional phase, the host cells “dissolved” and HPV particles containing cells are not necessarily vacuoles and other phenomena, suggesting that the virus can be released due to cell lysis and cause intraepithelial diffusion and Re-infection; vacuolar cells can not be used as a reliable basis for the diagnosis of the virus infection.