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目的:研究肝复康滴丸含药大鼠血清对CCl4损伤原代肝细胞的保护作用。方法:采用酶消化法分离大鼠肝实质细胞进行体外培养并建立CCl4肝损伤模型,以AST和ALT为指标,并采用MTT法对细胞活性和增殖进行检测,观察肝复康滴丸含药血清对肝损伤原代细胞的保护作用。结果:肝复康滴丸含药血清可使肝损伤大鼠原代细胞的增殖率明显提高,AST和ALT的含量明显降低。结论:肝复康滴丸具有很好的肝细胞保护作用。
Objective: To study the protective effect of rat serum containing Ganfukang Dripping Pill on primary hepatocytes damaged by CCl4. METHODS: Rat liver parenchymal cells were isolated by enzymatic digestion and cultured in vitro. CCl4 liver injury model was established. AST and ALT were used as indicators, and cell viability and proliferation were measured by MTT assay to observe the serum concentration of Ganfukang Dripping Pills. Protection of primary cells from liver injury. RESULTS: The serum containing Ganfukang Dripping Pills significantly increased the proliferation rate of primary cells in liver injury rats, and the content of AST and ALT decreased significantly. Conclusion: Ganfukang Dripping Pill has a good protective effect on liver cells.