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旨在以非肥胖糖尿病(Non-obese diabetic,NOD)小鼠为动物模型,研究高剂量昆虫细胞表达的重组热休克蛋白gp96(Recombinant gp96,rgp96)对1型糖尿病(Type 1 diabetes,T1D)的预防作用。以高剂量rgp96免疫NOD小鼠,用血糖仪监测小鼠血糖值,用流式细胞术检测小鼠脾脏CD4~+CD25~+Foxp3~+调节性T细胞(Regulatory T cells,Tregs)亚群频率,然后用一系列体外实验探究高剂量rgp96对Tregs的影响。结果显示高剂量rgp96免疫有效地预防或延缓小鼠T1D发病,免疫诱导Tregs数量明显增加。体外实验发现rgp96蛋白促进Tregs增殖,诱导Foxp3表达上调和IL-10分泌增加。研究结果为开发基于rgp96的新型T1D预防和治疗性疫苗提供了依据。
The aim of this study was to investigate the effect of recombinant gp96 (rgp96) on the expression of type 1 diabetes (T1D) in non-obese diabetic (NOD) mice. Preventive effect. The NOD mice were immunized with rgp96 at a high dose. The blood glucose level was monitored by blood glucose meter. The subsets of CD4 ~ + CD25 ~ + Foxp3 ~ + regulatory T cells (Tregs) were detected by flow cytometry , Then a series of in vitro experiments to explore the impact of high doses of rgp96 Tregs. The results showed that high doses of rgp96 immunization effectively prevent or delay the onset of T1D in mice, immune-induced Tregs increased significantly. In vitro experiments showed that rgp96 protein promoted Tregs proliferation, induced Foxp3 expression and increased IL-10 secretion. The results provide the basis for the development of a new type of T1D preventive and therapeutic vaccine based on rgp96.