通过对葡萄组织培养快速繁殖和微茎尖培养的接种、继代繁殖、生根及移栽等关键技术的研究,筛选出适于多种品种生长的广潜培养基.采用价格便宜的化学试剂等代用品配制培养基,使其成本降低46.3%至58.2%.其茎段培养方法已应用于繁殖珍贵的葡萄品种苗木,微茎尖培养将为培育无病毒苗提供技术准备. Through the study of the rapid propagation of grape tissue culture and the inoculation of the shoot tip culture, the secondary propagation, rooting and transplanting and other key technologies, the screening of suitable for a variety of varieties of wide-growth medium. The use of cheaper chemical reagents Substitutes formulated medium to reduce their costs by 46.3% to 58.2% .Its stem segment culture method has been applied to the breeding of precious grape seedlings, and the preparation of virus-free shoot tips will provide technical support for culturing virus-free seedlings.