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目的探讨细胞色素P4502E1(CYP2E1)基因多态性与酒精性肝病(ALD)的关系。方法将2004年10月至2005年10月兰州大学第一医院消化科住院及门诊患者80例分为ALD组(40例)和非ALD组(40例)以及健康体检中心103名体检者分为嗜酒组(40名)和正常组(63名)。ALD组与嗜酒组患者均饮酒>80g/d,连续10年以上。非ALD组和正常组均不饮酒。采用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测ALD组、非酒精性肝病组、嗜酒组、正常组中CYP2E1的基因多态性,并进行相关性分析。结果ALD组中C2等位基因型与对照组相比明显升高,P<0.001,差异有显著性意义;在ALD组中随肝纤维化指标异常程度增加,C2基因型明显升高,差异具有显著性意义,P<0.05。结论ALD与CYP2E1基因相关,CYP2E1等位基因C2可能是ALD的危险因素。
Objective To investigate the relationship between CYP2E1 gene polymorphism and alcoholic liver disease (ALD). Methods From October 2004 to October 2005, 80 inpatients and outpatients in the Department of Gastroenterology, First Hospital of Lanzhou University were divided into three groups: ALD group (40 cases) and non-ALD group (40 cases), and 103 physical examination centers Alcoholism group (40) and normal group (63). ALD group and alcohol group patients were drinking> 80g / d, for more than 10 years. Non-ALD group and normal group were not drinking. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of CYP2E1 gene in ALD, non-alcoholic liver disease, alcohol addiction, and normal controls. Results The genotype of C2 in ALD group was significantly higher than that in control group (P <0.001), and the difference was statistically significant. In ALD group, C2 genotype was significantly increased with the increase of abnormal liver fibrosis index Significant significance, P <0.05. Conclusion ALD is associated with CYP2E1 gene and CYP2E1 allele C2 may be a risk factor for ALD.