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目的:研究食管鳞癌患者外周血、肿瘤旁组织和肿瘤组织内Treg细胞的分布情况及其在肿瘤免疫中的作用。方法:分离食管鳞癌患者外周血、肿瘤旁组织及肿瘤组织内浸润淋巴细胞,采用流式细胞术分析Treg细胞占CD4+T细胞的比例。结果:食管鳞癌患者肿瘤组织内Treg细胞占CD4+T细胞的比例明显高于肿瘤旁组织及外周血,且与临床分期相关。结论:食管鳞癌的免疫微环境存在免疫抑制。
Objective: To study the distribution of Treg cells in peripheral blood, para-tumor tissues and tumor tissues of patients with esophageal squamous cell carcinoma and its role in tumor immunity. Methods: Peripheral blood lymphocytes, paracancer tissues and tumor infiltrating lymphocytes were isolated from patients with esophageal squamous cell carcinoma. The proportion of Treg cells in CD4 + T cells was analyzed by flow cytometry. Results: The proportion of Treg cells in CD4 + T cells in esophageal squamous cell carcinoma tissues was significantly higher than that in paracancerous tissues and peripheral blood, and was correlated with clinical stage. Conclusion: There is immunosuppression in esophageal squamous cell carcinoma immune microenvironment.