论文部分内容阅读
目的为了探察证实黑质-纹状体DA能神经通路阻断对大鼠纹状体Mor和Calb阳性神经元及其蛋白质表达的影响。方法借助6-OHDA注射阻断前脑内侧束建立PD模型;应用APO诱导旋转、握力测试、Morris水迷宫方法检测模型大鼠的行为学改变;应用免疫组化染色和蛋白印迹技术检测PD模型大鼠纹状体Mor和Calb神经元形态结构和蛋白水平的变化。结果①阿扑吗啡诱导模型大鼠出现健侧方向的旋转。模型大鼠抓握悬吊时间明显较正常组长(P<0.05),其寻找和发现平台的时间也明显较正常组长(P<0.05)。②模型大鼠纹状体Mor阳性区域的面积明显小于正常组(P<0.05),Calb阳性区域面积的变化不明显(P>0.05)。③模型大鼠纹状体Calb蛋白的表达水平明显低于正常组(P<0.05)。结论黑质-纹状体DA能神经通路阻断可能通过突触传递机制影响其突触后神经元的形态结构、蛋白表达、生理机能。
Objective To investigate the effects of blockade of DA neurotransmitter in substantia nigra and striatum on the expression of Mor and Calb positive neurons and their proteins in striatum of rats. Methods The PD model was established by injection of 6-OHDA into the medial forebrain bundle. The APO-induced rotation and grip strength test and Morris water maze test were used to detect the behavioral changes. The immunohistochemical staining and Western blotting were used to detect the PD model Morphological changes and protein levels of rat striatum Mor and Calb neurons. Results ① Apomorphine-induced rat model contralateral rotation. The grasping and suspending time of the model rats was significantly longer than that of the normal group (P <0.05), and the time for finding and finding the platform was also significantly longer than that of the normal group (P <0.05). ② The area of Mor positive area of striatum in model rats was significantly smaller than that in normal rats (P <0.05), while the area of Calb positive cells did not change significantly (P> 0.05). ③ The expression level of Calb protein in striatum in model rats was significantly lower than that in normal rats (P <0.05). Conclusion Blocking of DA neurons in the substantia nigra and striatum may affect the morphology, protein expression and physiology of the postsynaptic neurons through the synaptic transmission mechanism.