作者将肝素化的、用 Eagle 培养基作对倍稀释的血液加至7%聚蔗糖和10%Sodi-um metrizoate[注:是一种3-乙酰胺基-2,4,6-5(N-甲基乙酰胺基)苯甲酸盐]的混合液上,在4℃,300g 离心30分钟分离狒狒和人的单核细胞,用含灭活小牛血清的 Eagle液配成每毫升含2. 5×10~6单核细胞的悬液,加入平底微量细胞培养板,每个凹井内加0. 2毫升,37℃培养3小时后,洗去未粘附的细 The authors applied heparinized, double-diluted blood to Eagle’s medium to 7% Ficoll and 10% Sodi-um metrizoate [Note: a 3-acetamido-2,4,6-5 (N- Methylacetamido) benzoate], baboon and human monocytes were isolated by centrifugation at 300 g for 30 minutes at 4C and equilibrated with Eagle’s fluid containing inactivated calf serum to make 2 per ml. 5 × 10 ~ 6 mononuclear cell suspension, adding flat trace amount of cell culture plate, add 0.2 ml of each well, after 37 ° C for 3 hours, wash away the non-adherent fine