论文部分内容阅读
Bt Cry毒素广泛应用于害虫防治,但存在杀虫谱窄、活性低、靶标害虫易产生抗性等缺点。为了弥补这些不足,采取适当措施增强Cry毒素的杀虫作用十分必要。本文围绕Cry毒素的作用机理,论述了利用丝氨酸蛋白酶抑制剂、几丁质酶、增效蛋白、钙粘蛋白片段和Cyt毒素等增效因子提高Cry毒素的杀虫活性,延缓昆虫抗性的研究进展;探讨了利用基因定点突变、蛋白融合和杂交以及晶体蛋白末端小片段的去除等分子技术手段对毒素蛋白进行遗传改良,改善Cry毒素的杀虫性能,扩大其杀虫范围。
Bt Cry toxins are widely used in pest control, but there are some shortcomings of insecticidal spectrum, low activity and resistance to target pests. In order to make up for these deficiencies, it is necessary to take appropriate measures to enhance the insecticidal effect of Cry toxins. This article focuses on the mechanism of action of Cry toxins, and discusses the use of serine protease inhibitors, chitinase, booster protein, cadherin fragments and Cytotoxins and other synergistic factors to enhance the insecticidal activity of Cry toxin and delay insect resistance The genetic improvement of toxin protein was studied using molecular techniques such as site-directed mutagenesis, protein fusion and hybridization, and the removal of the small fragment of the crystal protein to improve the insecticidal properties of Cry toxins and to broaden their pesticidal range.