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目的探讨BMSCs对乙酸胃溃疡愈合质量的影响和机制。方法取清洁级雄性Wistar大鼠48只,用乙酸诱导大鼠胃溃疡模型,造模后第3天随机分为3组,每组16只。A组仅打开腹腔,拉出胃;B、C组打开腹腔拉出胃后,在溃疡周围胃壁浆膜下5个不同点分别注入150μL PBS和150μL第4代BMSCs PBS液(细胞密度为1×108个/100μL)。第10天测量溃疡面积,组织学方法检测再生胃黏膜厚度和扩张腺体数,免疫组织化学染色方法检测溃疡边缘VEGF的表达。结果 C组溃疡面积显著低于A、B组(P<0.01);A、B组间比较差异无统计学意义(P>0.05)。HE染色示,C组溃疡边缘再生胃黏膜较A、B组厚,扩张的腺体较少,黏膜结构较规则。C组再生胃黏膜厚度显著厚于A、B组(P<0.01),扩张腺体数显著少于A、B组(P<0.01);A、B组间比较差异均无统计学意义(P>0.05)。免疫组织化学染色示,C组溃疡边缘胃黏膜VEGF阳性表达显著多于A、B组。C组VEGF表达积分吸光度(IA)值显著高于A、B组(P<0.01);A、B组间比较差异无统计学意义(P>0.05)。结论 BMSCs通过分泌VEGF加速胃溃疡愈合,提高溃疡愈合质量。
Objective To investigate the effect and mechanism of BMSCs on healing quality of gastric ulcer in acetic acid. Methods Forty-eight clean male Wistar rats were induced with acetic acid to induce gastric ulcer in rats. The rats were randomly divided into 3 groups (n = 16). Group A and group C only opened the abdominal cavity and pulled out the stomach. After opening the abdominal cavity and opening the stomach in group B and group C, 150 μL of PBS and 150 μL of the fourth generation BMSCs PBS solution (cell density 1 × 108/100 μL). The ulcer area was measured on the 10th day. The thickness of the regenerated gastric mucosa and the number of dilated glands were detected by histological method. The expression of VEGF at the ulcer edge was detected by immunohistochemical staining. Results The ulcer area in group C was significantly lower than that in group A and B (P <0.01). There was no significant difference between group A and group B (P> 0.05). Hematoxylin and eosin staining showed that the regenerated gastric mucosa at the edge of ulcer in group C was thicker than that of group A and group B, with fewer dilated glands and regular mucosal structure. The thickness of regenerated gastric mucosa of group C was significantly thicker than that of group A and B (P <0.01), and the number of dilated glands was significantly less than that of group A and B (P <0.01). There was no significant difference between groups A and B > 0.05). Immunohistochemical staining showed that the positive expression of VEGF in gastric ulcer in group C was significantly more than that in groups A and B. The integral absorbance (IA) value of VEGF expression in group C was significantly higher than that in group A and B (P <0.01). There was no significant difference between group A and group B (P> 0.05). Conclusion BMSCs can accelerate the healing of gastric ulcer and enhance the quality of ulcer healing by secreting VEGF.