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目的探讨肝星状细胞（ＨＳＣ）的活化与细胞间粘附分子１（ＩＣＡＭ１）表达的关系．方法用链酶蛋白酶和胶原酶原位灌流，Ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠ＨＳＣ，并进行体外培养，应用免疫组织化学方法观察静息或活化状态下的ＨＳＣ中ＩＣＡＭ１表达．结果静息的ＨＳＣ不表达ＩＣＡＭ１，而活化的ＨＳＣ表达ＩＣＡＭ１，且随着培养时间的延长ＩＣＡＭ１表达量逐渐增加．结论ＩＣＡＭ１表达与ＨＳＣ活化及肝纤维化的发生有关．
Objective To investigate the relationship between the activation of hepatic stellate cells (HSC) and the expression of intercellular adhesion molecule1 (ICAM1). Methods HSC was isolated by in situ perfusion with streptavidin and collagenase and centrifuged by Nycodenz density gradient centrifugation. The HSCs were cultured in vitro and the expression of ICAM-1 in resting or activated HSCs was observed by immunohistochemistry. Results resting HSC does not express ICAM 1, and activated HSC expression ICAM 1, and with the extension of culture time ICAM 1 expression increased. Conclusion The expression of ICAM-1 is related to the activation of HSC and the occurrence of liver fibrosis.