论文部分内容阅读
目的 研究国产东亚钳蝎 ( Buthusmartensi Karsch,Bm K)毒素多肽的纯化单体组份 Bm K AS- 1对大鼠背根神经节 ( DRG)神经元河豚毒素不敏感 ( TTX- R)钠电流的作用。方法 采用膜片箝全细胞记录方法 ,观察 Bm K AS- 1对单个DRG细胞 TTX- R钠电流幅值的影响。结果 Bm K AS- 1剂量依赖地抑制 TTX- R钠电流 ,且高剂量 ( 10 μg/m l) Bm K AS- 1几乎完全抑制 TTX- R钠电流 ,其抑制作用不可逆。结论 Bm K AS- 1显著抑制 TTX- R钠电流 ,这种抑制作用可能是其镇痛作用的机理之一
Objective To investigate the effect of Bm K AS-1, a purified monomeric component of domestic Buthus martensii Karsch (Bm K) toxin peptide, on tetrodotoxin-insensitive (TTX-R) sodium currents in rat DRG neurons effect. Methods Whole-cell patch-clamp recording was used to observe the effect of Bm K AS-1 on TTX-R sodium current amplitude in a single DRG cell line. Results Bm K AS-1 dose-dependently inhibited TTX-R sodium current, and high dose (10 μg / ml) Bm K AS-1 almost completely inhibited TTX-R sodium currents, and its inhibitory effect was irreversible. Conclusions Bm K AS-1 significantly inhibits TTX-R sodium currents, and this inhibition may be one of the mechanisms of its analgesic effect