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流行病学和实验室研究表明,5和8型金黄色葡萄球菌荚膜多糖(CP)既是毒力因子,又是保护性抗原。对菌血症患者的调查表明,5和8型占分离菌株的80％。研究发现,5和8型CP的特性与肺炎球菌、脑膜炎球菌及b型流感杆菌等的荚膜病原体相似,其CP分子量较小,不能作为有效的免疫原。结合菌苗在健康和免疫损害的成人中的免疫原性比多糖强。为此,作者采用绿脓杆菌外毒素A(ETA)作为载体蛋白,建立了分别与5和8型金黄色葡萄球菌CP合成结合菌苗的方法。选择ETA是因为这种蛋白与金黄色葡萄球菌无血清学关系,并证明抗ETA抗体能提供抗绿脓杆菌的防御作用。在幼鼠中,结合菌苗比CP具有更强的免疫原性,能诱导促进调理作用的型特异性抗体和抗ETA的
Epidemiological and laboratory studies have shown that S. aureus capsular polysaccharides (CP) types 5 and 8 are both virulence factors and protective antigens. A survey of patients with bacteremia showed that type 5 and 8 accounted for 80% of isolates. The study found that the properties of CPs 5 and 8 are similar to those of capsular pathogens such as pneumococci, meningococci and influenza b, and their CP molecular weight is small and can not be used as an effective immunogen. Binding vaccines are more immunogenic than polysaccharides in healthy and immunocompromised adults. To this end, the authors used Pseudomonas exotoxin A (ETA) as a carrier protein, and established a method for the synthesis of a combined vaccine with Staphylococcus aureus type 5 and 8, respectively. ETA was chosen because of its serological-less relationship with Staphylococcus aureus and demonstrates that anti-ETA antibodies provide a protection against Pseudomonas aeruginosa. In young mice, the binding bacterin is more immunogenic than CP and can induce type-specific antibodies that promote opsonization and anti-ETA