论文部分内容阅读
目的:研究姜黄素的抗增殖作用是否依赖于其对Ets-1表达的下调。方法:使用Ets-1 siRNA对CFs细胞Ets-1基因进行沉默;Real-time PCR和western-blot法测定各组细胞Ets-1 mRNA和蛋白的表达水平。结果:在mRNA和蛋白水平上,AngⅡ明显增加CFs细胞内Ets-1的表达;使用siRNA技术对Ets-1进行沉默后,随着Ets-1表达的降低及转录调节能力的下降,由AngⅡ诱导的CFs细胞的增殖能力及增殖相关细胞因子分泌减少;姜黄素可以显著降低AngⅡ诱导的Ets-1表达升高,并具有浓度依赖性(P<0.05)。说明姜黄素对AngⅡ诱导的CFs增殖的抑制作用可能依赖于其对Ets-1表达的抑制作用。结论:Ets-1 siRNA对Ets-1进行基因沉默后,显示出抗增殖效用;姜黄素能够有效地抑制AngⅡ诱导的CFs细胞Ets-1 mRNA和蛋白的过表达;姜黄素的抗增殖作用可能依赖于其对Ets-1基因的表达下调而得以实现的。
Objective: To investigate whether the antiproliferative effects of curcumin depend on its downregulation of Ets-1 expression. Methods: Ets-1 siRNA was used to silence Ets-1 gene in CFs cells. Real-time PCR and western blot were used to determine the expression of Ets-1 mRNA and protein. Results: At the mRNA and protein levels, AngⅡ significantly increased the expression of Ets-1 in CFs cells. After siRNA-mediated silencing of Ets-1, the expression of Ets-1 was down-regulated and the transcriptional regulation was reduced by AngⅡ (P <0.05). Curcumin significantly decreased AngⅡ-induced Ets-1 expression in a concentration-dependent manner (P <0.05). Curcumin on Ang Ⅱ induced CFs proliferation may be dependent on its inhibition of Ets-1 expression. Conclusion: Ets-1 siRNA shows anti-proliferative effect after Ets-1 gene silencing. Curcumin can effectively inhibit Ets-1 mRNA and protein overexpression induced by AngⅡ in CFs. The anti-proliferative effect of curcumin may be dependent on This is achieved by its downregulation of the expression of the Ets-1 gene.