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AIM: To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.METHODS: mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCRELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine.RESULTS: T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels,and telomerase activity was significantly increased or decreased.CONCLUSION: The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.