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目的通过地塞米松对白介素－５（ＩＬ－５）诱导的ＥＯＳ存活的影响，探讨地塞米松治疗哮喘的作用机制。方法将外周血的ＥＯＳ加入ＩＬ－５和不同浓度的ＤＥＸ共同培养，以观察ＤＥＸ对ＩＬ－５诱导的ＥＯＳ存活的作用。结果大于５ｐｇ／ｍｌ水平的ＩＬ－５明显提高ＥＯＳ的存活，且有剂量依赖性；Ｄｅｘ以剂量依赖的方式抑制ＥＯＳ的存活，从５０ｎｍｏｌ／Ｌ开始，１０００ｎｍｏｌ／Ｌ的Ｄｅｘ抑制达到最强；而当ＩＬ－５浓度为１００ｐｇ／ｍｌ或更高时，其诱导的ＥＯＳ存活不能被任何浓度的ＤＥＸ所抑制。Ｄｅｘ对ＥＯＳ存活的作用有时间依赖性，其抑制作用在高浓度的ＩＬ－５存在条件下可延迟或消失。结论Ｄｅｘ对疾病的治疗可能存在着抵抗，而高浓度的ＩＬ－５可抵消Ｄｅｘ对细胞的作用
Objective To investigate the effect of dexamethasone on the survival of EOS induced by interleukin-5 (IL-5) in asthmatic rats. Methods Peripheral blood EOS was added to IL-5 and cultured with different concentrations of DEX to observe the effect of DEX on IL-5-induced EOS survival. Results IL-5 levels above 5pg / ml significantly increased the survival of EOS in a dose-dependent manner. Dex inhibited the survival of EOS in a dose-dependent manner. From 50nmol / L, Dex inhibited 1000nmol / L, When IL-5 concentration was 100 pg / ml or higher, its induced EOS survival could not be inhibited by any concentration of DEX. Dex has a time-dependent effect on the survival of EOS, and its inhibitory effect can be delayed or disappeared in the presence of high concentrations of IL-5. Conclusions Dex may have resistance to disease treatment, whereas high concentrations of IL-5 counteract the effect of Dex on cells