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目的:探讨荔枝核总黄酮(TFL)对急性肺损伤大鼠胆碱能抗炎通路的影响。方法:将60只SD大鼠随机分成6组:空白对照组、模型对照组、地塞米松3 mg·kg~(-1)组、荔枝核总黄酮100,50,25 mg·kg~(-1)组,每组10只大鼠,连续灌胃给药7 d。末次给药30 min后,腹腔注射20%酵母混悬液(5 mL·kg~(-1))建立大鼠急性肺损伤模型。末次注射2 h后,处死大鼠,摘眼球取血法取血,分离出血清,待测。测定大鼠左肺组织进行的湿干比重(W/D)。测定肺组织中乙酰胆碱转移酶(ChAT)、乙酰胆碱酯酶(AChE)的活性。ELISA法检测血清中白介素~(-1)β(IL-1β)、一氧化氮(NO)、乙酰胆碱(ACh)、肿瘤坏死因子-α(TNF-α)的含量。蛋白免疫印迹(Western blot)法测定大鼠肺组织中核因子-κB(NF-κB)的蛋白含量。结果:与空白对照组相比,模型组大鼠湿干比重上升,IL-1β、NO、TNF-α及NF-κB的含量提高,ACh的含量下降,ChAT和AChE的活性减弱(P<0.05)。与模型组相比,荔枝核总黄酮组的大鼠肺组织湿干比重下降,IL-1β、NO、TNF-α及NF-κB的含量下降,ACh的含量升高,ChAT和AChE的活性增加(P<0.05)。结论:荔枝核总黄酮可通过调节胆碱能抗炎通路,抑制炎症反应的发展,使受损肺组织得到保护。
Objective: To investigate the effect of total flavonoids of litchi nucleus (TFL) on cholinergic anti-inflammatory pathway in rats with acute lung injury. Methods: Sixty SD rats were randomly divided into 6 groups: blank control group, model control group, dexamethasone 3 mg · kg -1 group, total flavones of litchi kernel 100, 50, 25 mg · kg ~ (- 1) group, each group of 10 rats, continuous intragastric administration of 7 d. Thirty minutes after the last administration, a rat model of acute lung injury was established by intraperitoneal injection of 20% yeast suspension (5 mL · kg -1). 2 h after the last injection, rats were sacrificed, blood was taken from the eye to take blood, serum was separated and tested. The left and right lungs of rats were weighed and wet-dry weight (W / D) was measured. The activities of acetylcholinesterase (ChAT) and acetylcholinesterase (AChE) in lung tissue were determined. Serum levels of IL-1β, NO, ACh and TNF-α were detected by ELISA. Protein content of nuclear factor-κB (NF-κB) in rat lung tissue was determined by Western blot. Results: Compared with the blank control group, the wet weight and dry weight of the model group increased, the content of IL-1β, NO, TNF-α and NF-κB increased, the content of ACh decreased and the activity of ChAT and AChE decreased ). Compared with the model group, the lung tissue of litchi nuclear total flavonoids decreased the wet weight and dry weight, the content of IL-1β, NO, TNF-α and NF-κB decreased, the content of ACh increased and the activity of ChAT and AChE increased (P <0.05). Conclusion: The total flavonoids of litchi nucleus can protect the damaged lung tissue by regulating the cholinergic anti-inflammatory pathway and inhibiting the development of inflammatory reaction.