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目的:研究黄芪多糖对辐照引起的放射性肺损伤小鼠TGF-β1、SOD的影响,以了解黄芪对放射性肺损伤抑制作用机制。方法:近交系C57/BL雌性小鼠120只,随机数字表法分为4组:对照组、单纯照射组、照射+黄芪多糖组(100 mg/kg·d),单纯黄芪多糖组(100 mg/kg·d);每组30只小鼠。照射方式:10 MV-X射线,经前胸单次照射小鼠肺脏中平面13Gy,全肺照射。给药方式:灌胃,照射前30 min给药1次,以后每日给药至实验结束,对照组给予相应同体积生理盐水。在照射后7 d、28 d、56 d处死后予以肺泡灌洗液(BALF)提取计数炎性细胞;另取部分肺组织HE染色,部分肺组织行超氧化物歧化酶(SOD)检测,ELISA法测定血清中TGF-β1。结果:未被照射组别肺组织均无炎症反应。受照射组(单纯照射组、照射+黄芪多糖组)在照射后56 d均表现出不同程度的放射性肺炎病理变化,但照射+黄芪多糖组较单纯照射组炎症反应明显较轻,照射+黄芪多糖组与同时段的单纯照射组比较:肺组织中血清中TGF-β1明显降低,SOD水平明显较高,炎性细胞募集较少。两组间不同时段血清中TGF-β1水平、肺组织中SOD水平以及炎性细胞计数均具有明显差异(P<0.05或P<0.01)。结论:黄芪多糖在放射性肺损伤小鼠模型中通过保护SOD,降低TGF-β1,减轻炎症反应,起到减轻小鼠放射性肺损伤的作用。
Objective: To study the effect of Astragalus Polysaccharides on the expression of TGF-β1 and SOD in radiation-induced lung injury in mice, in order to understand the mechanism of astragalus on radiation-induced lung injury. Methods: 120 female C57 / BL mice were randomly divided into 4 groups: control group, irradiation group, irradiation + APS group (100 mg / kg · d), APS group mg / kg · d); 30 mice per group. Irradiation mode: 10 MV-X ray, single plane thoracic radiation in the thoracic plane 13Gy, whole lung irradiation. Mode of administration: gavage, administration 30 min before irradiation once, after daily administration to the end of the experiment, the control group given the same volume of saline. After 7 days, 28 days and 56 days after irradiation, bronchoalveolar lavage fluid (BALF) was taken for counting and counting of inflammatory cells. In addition, some of the lung tissues were stained with HE, some of the lung tissues were detected by superoxide dismutase (SOD), ELISA Determination of serum TGF-β1. Results: There was no inflammatory reaction in the non-irradiated lung tissue. The pathological changes of radiation pneumonitis showed different degrees on the 56th day after irradiation in the irradiated group (irradiation alone, irradiation + astragalus polysaccharide group), but the radiation reaction + astragalus polysaccharide group was significantly lighter than the irradiation alone group, the radiation + astragalus polysaccharide Group compared with the same period of simple irradiation group: lung tissue TGF-β1 was significantly lower, SOD was significantly higher, less inflammatory cell recruitment. Serum levels of TGF-β1, SOD in lung tissue and inflammatory cell counts at different time points were significantly different between the two groups (P <0.05 or P <0.01). Conclusion: APS plays an important role in alleviating radiation-induced lung injury in mice with radiation-induced lung injury through protecting SOD, decreasing TGF-β1 and decreasing inflammation.