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探讨神经节苷脂GM1对新生缺氧缺血性脑损伤大鼠神经的保护作用,本文对新生缺氧缺血性脑损伤(HIBD)大鼠用GM1治疗,并与假手术组和未治疗组对照,观察GM1对新生HIBD大鼠体重增长、脑重量、翻身、夹角尖叫等行为的影响,并通过大鼠游走水迷宫实验观察GM1治疗对其年长后学习和记忆的影响。结果显示:(1)实验后48h三组动物体重即已有差别,以HIBD组最低,对照组最高,随着日龄的增加体重的差别越来越明显。至42天以HIBD组体重最低,GM1治疗组和对照组相近;(2)对HIBD 48小时左右两脑半球重量测定显示,HIBD组右脑明显重于左脑,提示有脑水肿存在,72小时至42天,右脑重量则明显减轻,提示有脑萎缩存在;GM1治疗组48小时右脑也明显重于左脑,但72小时至42天两侧半球的重量无差异;(3)实验24小时内大鼠神经行为异常发生率以HIBD组最高,其次是GM1治疗组,对照组最低;48小时后GM1治疗组神经行为异常的发生率明显低于HIBD组,与对照组相比无差异。大鼠游走出水迷宫的时间以正常对照组游走有规律且时间短,GM1治疗组与正常对照组相比无统计学差异,而HIBD组大鼠走出迷宫时间较治疗组及对照组明显延长。结论:神经节苷脂GM1可以减少HIBD后的体重下降,使体重增长达正常水平。还可以减轻脑水肿和惊厥的发生,促进脑损伤的恢复,并增加HIBD大鼠
To investigate the neuroprotective effect of ganglioside GM1 on neonatal rats with hypoxic-ischemic brain damage. In this study, GM1 was administered to neonatal hypoxic-ischemic brain damage (HIBD) rats and compared with sham-operated and untreated To observe the effects of GM1 on body weight gain, brain weight, stand-up and screaming in newborn HIBD rats. The effect of GM1 on learning and memory of elderly rats after GM1 treatment was observed by swimming maze test in rats. The results showed that: (1) At 48h after the experiment, the body weight of the three groups of animals had been different, with the lowest in HIBD group and the highest in control group. The weight difference became more and more obvious with the increasing of age. The body weight of the HIBD group was the lowest at 42 days, and the GM1 treatment group was similar to that of the control group. (2) The weight of both hemispheres of HIBD group at 48 hours showed that the right brain of HIBD group was significantly heavier than the left brain, At 42 days, the weight of the right brain was significantly reduced, suggesting that there was brain atrophy. In the GM1-treated group, the right hemisphere was significantly heavier than the left hemisphere at 48 hours, but there was no difference in the weights of hemispheres between 72 and 42 days. (3) Experiment 24 The incidence of neurobehavioral abnormalities in rats in the first hour was the highest in HIBD group, followed by that in GM1 group and the lowest in control group. After 48 hours, the incidence of neurobehavioral abnormalities in GM1 group was significantly lower than that in HIBD group. Rats walked out of the water maze time to normal control group walked a short time, GM1 treatment group compared with the normal control group was no significant difference, while HIBD rats out of the maze time than the treatment group and the control group was significantly longer . Conclusion: Ganglioside GM1 can reduce the weight loss after HIBD, so that the weight gain up to normal levels. Can also reduce the occurrence of cerebral edema and convulsion, promote the recovery of brain injury, and increase HIBD rats