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为了检测单侧注射ＭＰＴＰ制备的帕金森病恒河猴模型纹状体内多巴胺及其代谢产物的含量变化，本研究采用脑内微透析技术和高效液相色谱－ 电化学方法检测了双侧尾状核头部多巴胺及其代谢产物３，４－二羟基苯乙酸和高香草酸的含量。结果证明，ＭＰＴＰ注射侧与注射对侧相比，多巴胺、３，４－二羟基笨乙酸和高香草酸的含量分别降低８５．７％ ， ９５．１％ 和６７．８％ 。这与动物呈现单侧帕金森病症状，核磁共振检查显示单侧黑质区域密度减低、面积缩小以及经抗酪氨酸羟化酶免疫组化方法显示脑切片单侧多巴胺能神经元明显减少等现象一致。以上结果表明，脑内微透析技术是活体检测脑内神经递质含量变化的有效方法，可以反映模型的病程变化，可用于评价帕金森病的治疗效果。
In order to detect dopamine and its metabolites in the striatum of Parkinson’s disease (Rhesus monkey) model of Parkinson’s disease (MPTP) prepared by unilateral injection of MPTP, we used intracerebral microdialysis technique and high performance liquid chromatography-electrochemistry Contents of nuclear dopamine and its metabolites 3,4-dihydroxyphenylacetic acid and homovanillic acid. The results demonstrated that dopamine, 3,4-dihydroxyphenylacetic acid and homovanillic acid levels decreased by 85.7%, 95.1%, and 67.8%, respectively, in the MPTP injection side compared to the contralateral side. This animals showed unilateral Parkinson’s disease symptoms, MRI showed reduced density of unilateral substantia nigra region, area reduction and anti-tyrosine hydroxylase immunohistochemical method showed a significant reduction in brain slices unilateral dopaminergic neurons The same phenomenon. The above results show that intracerebral microdialysis technique is an effective method to detect changes of neurotransmitter in the brain, which can reflect the changes of the course of the model and can be used to evaluate the therapeutic effect of Parkinson’s disease.