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目的:通过对研究脐带间充质干细胞(Umbilical cord mesenchymal stell cells,UCMSCs)与人恶性胶质母细胞瘤细胞U87MG细胞(U87 Malignant glioma cells)体外共培养,模拟肿瘤生长的内环境,以及其对U87MG细胞增值作用的影响及肿瘤细胞与间充质干细胞的共培养方法。方法:提取人脐带间充质干细胞进行体外培养、扩增,用MTT法测定UMSCS上清液对U87MG的影响,用瑞士染色法检测U87MG形态学变化。结果:MTT比色法结果显示UMSCS对U87MG有抑制作用。96小时培养后1:8、1:4、1:2及未稀释的UMSCs上清液对U87MG的抑制率分别为17%,24%,37.2%及46.4%,U87MG细胞形态亦随着培养时间的延长由多角形变为梭形,突起消失,细胞间骨架结构断裂。结论:通过对共培养前后U87MG与UMSCs共培养后形态学变化、生长曲线变化及对生长周期的影响作用的观察分析,得出UMSCs及其上清液对U87MG有抑制作用,而且呈时间及浓度依赖性。
OBJECTIVE: To simulate the internal environment of tumor growth through the in vitro co-culture of umbilical cord mesenchymal stem cells (UCMSCs) with human malignant glioblastoma U87MGG cells (U87Magnantant glioma cells) U87MG cell proliferation and tumor cells and mesenchymal stem cells co-culture method. Methods: Human umbilical cord mesenchymal stem cells were isolated and cultured in vitro. The effects of UMSCS supernatant on U87MG were detected by MTT assay. The morphological changes of U87MG were detected by Swiss staining. Results: MTT colorimetric assay showed that UMSCS inhibited U87MG. The inhibitory rates of U87MG on 1: 8, 1: 4, 1: 2 and undifferentiated UMSCs supernatants after culture for 96 hours were 17%, 24%, 37.2% and 46.4%, respectively. The extension of the polygons into fusiform, protrusions disappear, the cell skeleton structure rupture. Conclusion: Through the co-culture of U87MG before and after co-culture morphological changes, changes in the growth curve and the impact of the growth cycle observation and analysis, that UMSCs and supernatant of U87MG inhibitory effect, and the time and concentration Dependency.