论文部分内容阅读
Long-term exposure of nitrifiers to high concentrations of free ammonia (FA) and free nitrous acid (FNA) may affect nitrifiers activity and nitrous oxide (N2O) emission.Two sequencing batch reactors (SBRs) were operated at influent ammonium nitrogen (NH-4-N) concentrations of 800 mg/L (SBRH) and 335 mg/L (SBRL),respectively.The NH4-N removal rates in SBRH and SBRL were around 2.4 and 1.0 g/L/day with the nitritation efficiencies of 99.3％ and 95.7％,respectively.In the simulated SBR cycle,the N2O emission factors were 1.61％ in SBRH and 2.30％ in SBRL.N2O emission was affected slightly by FA with the emission factor of 0.22％-0.65％,while N2O emission increased with increasing FNA concentrations with the emission factor of 0.22％-0.96％.The dominant ammonia oxidizing bacteria (AOB) were Nitrosomonas spp.in both reactors,and their relative proportions were 38.89％ in SBRH and 13.36％ in SBRL.Within the AOB genus,a species (i.e.,operational taxonomic unit [OTU] 76) that was phylogenetically identical to Nitrosomonas europaea accounted for 99.07％ and 82.04％ in SBRH and SBRL,respectively.Additionally,OTU 215,which was related to Nitrosomonas stercoris,accounted for 16.77％ of the AOB in SBRL.