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背景与目的:代谢酶尿苷二磷酸葡糖醛酸转移酶1A7(UDP-glucuronosyltcansferase,UGT1A7)可催化香烟中的致癌物质苯并[琢]芘、亚硝胺NNK和杂环胺PhIP与葡糖醛酸结合使之失活,在解毒机制中起重要作用。本实验旨在研究UGT1A7基因多态与肺癌易感性的关系。方法:以聚合酶链反应-变性高效液相色谱(DHPLC)技术和聚合酶链反应-限制性片段长度多态性方法,分析317例正常对照和312例肺癌患者外周血淋巴细胞基因组DNA,UGT1A7129～131和208位点多态基因型分布,及其与肺癌风险的关系。结果:与携带UGT1A7*1/*1基因型个体比较,携带UGT1A7*3/*1基因型的个体患肺腺癌的风险增高1.80倍(校正的OR为1.80;95%CI1.03～3.12),携带UGT1A7*3基因型的个体患肺腺癌的风险增高1.59倍(校正的OR为1.59,95%CI0.96～2.63)。UGT1A7多态与肺鳞癌风险不相关。结论:UGT1A7基因多态可能是中国人肺癌遗传易感性因素。
BACKGROUND & OBJECTIVE: The metabolic enzyme uridine diphosphate glucuronosyltransferase 1A7 (UDP-glucuronosyltcansferase, UGT1A7) can catalyze the carcinogens benzo [Zhuo] pyrene, nitrosamines NNK and heterocyclic amines PhIP and glucose Deactivation of the combination of aldehyde and acid, in detoxification mechanism plays an important role. The purpose of this study was to investigate the relationship between UGT1A7 polymorphism and lung cancer susceptibility. Methods: The genomic DNA of peripheral blood lymphocytes from 317 healthy controls and 312 lung cancer patients were analyzed by polymerase chain reaction-denaturing high performance liquid chromatography (DHPLC) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). UGT1A7129 ~ 131 and 208 loci polymorphism genotype distribution and its relationship with the risk of lung cancer. RESULTS: Individuals with the UGT1A7 * 3 / * 1 genotype had a 1.80-fold increased risk of developing lung adenocarcinoma compared with individuals carrying the UGT1A7 * 1 / * 1 genotype (corrected OR 1.80; 95% CI 1.03 to 3.12) , Individuals with UGT1A7 * 3 genotype had a 1.59-fold increased risk of developing lung adenocarcinoma (adjusted OR, 1.59, 95% CI, 96 to 2.63). UGT1A7 polymorphism is not associated with the risk of squamous cell carcinoma of the lung. Conclusion: UGT1A7 gene polymorphism may be a genetic predisposition to lung cancer in Chinese.