目的研究肉苁蓉苯乙醇总苷对酪氨酸酶活性的抑制作用。方法用L-酪氨酸作为底物,测定药物干预前后酪氨酸酶的活性,计算酪氨酸酶的抑制率,并以Lineweaver-Burk双倒数法绘制酶动力学曲线,推断抑制类型。结果苯乙醇总苷对酪氨酸酶的活性具有明显下调作用,对酪氨酸酶的效应表现为竞争型可逆性抑制。结论本研究可为开发利用肉苁蓉中的美白成分提供依据。 Objective To study the inhibition of tyrosinase activity by glycosides of Cistanche tubulosa. Methods L-Tyrosine was used as a substrate to determine the tyrosinase activity before and after drug intervention. The tyrosinase inhibition rate was calculated. The enzyme kinetic curve was drawn by Lineweaver-Burk double reciprocal method to deduce the inhibition type. Results Phenylethanoside had a significant down-regulation of tyrosinase activity and a competitive reversible inhibition of tyrosinase. Conclusion This study can provide the basis for the development and utilization of whitening ingredients in Cistanche.