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采用cDNA末端快速扩增(rapid amplification of cDNA ends,RACE)方法得到了百子莲(Agapanthus praecox ssp.orientalis)FT基因cDNA全长序列,命名为ApFT,GenBank登录号为KC951108。序列分析表明,百子莲ApFT基因cDNA全长815 bp,其中开放阅读框534 bp,编码177个氨基酸,5′非编码区(5′UTR)和3′非编码区(3′UTR)分别为89 bp和192 bp。ApFT编码的蛋白有一个单一而非常保守的PEBP(Phosphatidylethanolamine-binding protein,PEBP磷酸乙醇胺结合蛋白)结构域,与玉米(MFT2)、拟南芥(AtFT)和温州蜜柑(CuMFT1)等植物的FT蛋白有较高的相似性,同源性均在50%以上。系统进化树分析表明,百子莲ApFT与玉米(MFT2)聚类关系最近。实时荧光定量qRT-PCR结果显示,整个花芽分化过程中ApFT在叶片中表达量高,茎尖中表达量低。随着花芽分化的进程,在叶片中ApFT表达量逐渐降低,而茎尖中ApFT的表达量在诱导期均高于营养期和花芽分化期,推测该基因可能在调节百子莲花芽分化过程中发挥重要作用。
The full length cDNA of FT gene of Agapanthus praecox ssp. Orientalis was obtained by rapid amplification of cDNA ends (RACE) method and named ApFT. The GenBank accession number was KC951108. Sequence analysis showed that the full-length cDNA of ApFT gene was 815 bp, including 534 bp open reading frame (ORF) encoding 177 amino acids, 5 ’UTR and 3’ UTR bp and 192 bp. The ApFT-encoded protein has a single and very conserved PEBP (Phosphatidylethanolamine-binding protein) domain, and FT proteins of plants such as maize (MFT2), Arabidopsis thaliana (AtFT) and Satsuma mandarin (CuMFT1) Has a high similarity, homology are more than 50%. Phylogenetic tree analysis showed that the relationship between Apitum miltiorrhiza ApFT and maize (MFT2) clustering was the closest. Real-time qRT-qRT-PCR results showed that the expression of ApFT was high in leaves and low in shoot tips during flower bud differentiation. With the process of flower bud differentiation, the expression of ApFT in leaves gradually decreased, while the expression of ApFT in shoot tip was higher than that in vegetative stage and flower bud differentiation stage in inducing stage, suggesting that this gene may play an important role in regulating flower bud differentiation Play an important role.