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提高共生固氮作用方法之一是增强寄主植物的固氮潜力。弗吉尼亚的花生(落花生Arachis hypcgacal)品种(密枝亚种ssp.hypogale密枝变种Var hypogala)和西班牙(spanish)类型(疏枝亚种ssp.fastigiataWaldron var.vulgaris.Harz)品种的固氮能力不同.本试验的目的是测定一个从弗吉尼亚品种和一个西班牙品种杂交所得的高代杂种中的固氮指标性状的变异和相互关系.由弗古尼亚品种、NC_(?)和西班牙选系922杂交得来的F_5和F_6代中的30个F_2家系的固氮活力指示性状的变异作了研究.试验分两年在两地进行,试验中取样三次,分别测定亲本品系和F_2家系的根瘤数,根瘤重用乙炔还原法测定固氮酶活性,专化固氮酶活性及茎叶重。果重在成熟期最后取样测定。品种NC_6,除专化固氮酶活性外,所有测定性状都优于922品系.各家系中观察到大多数性状都存在着显著的差异。根瘤数、根瘤重、固氮酶活性,茎叶重及果重的广义遗传力估算从中到高,这就表明在这样的群体中优良的基因型是能够而且容易被鉴定出.根瘤数和根瘤重相互间和固氮酶性间均呈正相关,这就表明仅仅测定固氮酶即可鉴定优良家系.阅氮酶活性的表现型和基因型相关同茎叶重的关系都显著,在各次取样期及其环境条件下,其基因型的相关系数的范围从0.66—0.89.但是,固氮酶活性和产量的相关值较小(F_5为0.36,F_6为0.57,世代间为0.47),这表明固氮酶活性对荚果重的影响比对茎叶重要小。因此,选择具有较大固氮能力的家系是可能的,而且将导致对产量的间接选择.
One of the ways to increase symbiotic nitrogen fixation is to increase the nitrogen fixation potential of host plants. The varieties of Virginia peanut (Arachis hypcgacal) (Var hypogala ssp.hypogale) and Spanish spanish (Ssp.fastigiataWaldron var.vulgaris.Harz) were different in their ability to fix nitrogen The purpose of the experiment was to determine the variation and correlation of nitrogen fixation traits in a cross between a Virginia variety and a Spanish cross. The crosses between the virginia variety NC_ (?) And the Spanish line 922 F_5 and F_6 generations of 30 F_2 nitrogen fixation activity indicated variation of the test was carried out in two years in two places, the sample was sampled three times to determine the parental line and F_2 pedigree nodules, nodules re-use Determination of nitrogenase activity by acetylene reduction method, nitrogenase activity and leaf weight. Fruit weight at the end of the mature sample determination. Variety NC_6, except for the specialization of nitrogenase activity, showed that all the tested traits were better than 922. Most of the traits were observed significant differences in most traits. The general heritability of nodule weight, nodule weight, nitrogenase activity, stem-leaf weight, and fruit weight is estimated from medium to high, indicating that good genotypes in such populations are able and easily identified. The number of nodules and the nodule weight There was a positive correlation between nitrogenase activity and nitrogenase activity, which indicated that only the determination of nitrogenase enzyme could identify elite families.The phenotypic and genotypic correlation of nitrogenase activity with stem weight was significant, and in each sampling period and The correlation coefficient of genotypes ranged from 0.66 to 0.89 under environmental conditions, however, the correlation between nitrogenase activity and yield was small (F_5 0.36, F_6 0.57 and 0.47 between generations), indicating that nitrogenase activity The effect on pod weights is less important than stems and leaves. Therefore, it is possible to choose a pedigree with greater nitrogen fixation capacity, and will result in an indirect choice of production.