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目的 探讨中国北方汉族人群Kalrn基因rs9289231单核苷酸多态性与冠状动脉粥样硬化性疾病(coronary artery disease,CAD)发病的相关性.方法 选取2015年6月-2016年5月在中国人民武装警察部队总医院心内科住院的拟诊断冠心病患者286例,其中234例经冠脉造影证实有冠心病的患者作为A组,另52例行冠状动脉造影排除冠心病的患者作为B组.采用血液DNA试剂盒提取两组患者外周静脉血DNA,并通过聚合酶链式反应-连接酶检测反应(PCR-LDR)技术检测rs9289231位点基因型,分析Kalrn基因rs9289231单核苷酸多态性与冠状动脉粥样硬化性疾病(CAD)相关性.结果 Kalrn基因rs9289231在A、B两组中均存在TT、GT和GG 3种基因型,且基因分型符合Hardy-Weinbery平衡;A组中3种基因型分布频率及等位基因分布频率与B组比较,差异均无统计学意义(P＞0.05);采用Binary Logistic回归分析校正冠心病其他易患危险因素后,仍提示Kalrn基因rs9289231多态性与CAD的发病无相关性.结论 Kalrn基因rs9289231多态位点与中国北方汉族人群冠心病发病可能无相关性.“,”Objective To investigate the relationship between Kalm gene rs9289231 polymorphism and coronary artery disease (CAD)in Northern Chinese Han population.Methods Peripheral venous blood samples were collected from 234 inpatients with CAD confirmed by coronary angiography (Group A)and 52 inpatients without CAD (Group B).Polymerase chain reaction-ligase detection reaction (PCR-LDR)technology was used to identify the Kalrn gene polymorphisms.Results Three genotypes:TT,GT,and GG were found in the Kalrn gene rs9289231 in both Groups A and B,and the genotyping conformed to the Hardy-Weinbery balance.There were not sigificant differences in the distribution frequencies of these 3 genotypes and alleles between Group A and Group B (all P ＞0.05).Binary logistic regression analysis was adopted to adjust multiple risk factors and showed that there was still no significant correlation between Kalrn gene rs9289231 polymorphism with CAD.Conclusion The Kalrn gene rs9289231 polymorphism doesn" t have correlation with CAD in Northern Chinese Han population.