目的:优选聚酰胺纯化薄荷总黄酮的工艺条件。方法:以总黄酮含量和吸附-洗脱率为指标,采用单因素试验考察5种不同类型的纯化材料对总黄酮的吸附能力,确定纯化材料,并对其纯化工艺中吸附条件、除杂条件和洗脱条件进行优化。结果:优化工艺条件为上样液质量浓度0.1 g.mL-1,上样量为生药量与聚酰胺体积比1∶6,吸附流速2 BV.h-1,柱床径高比1∶9,待吸附结束后,用3 BV 10%乙醇洗脱除杂,除杂流速4 BV.h-1,再用3 BV 90%乙醇以8 BV.h-1流速洗脱,经聚酰胺(30～60目)纯化后,薄荷总黄酮纯度可达50%。结论:优选的纯化工艺分离效果良好、操作简单、重复性好,可用于分离富集薄荷总黄酮。 OBJECTIVE: To optimize the conditions for the purification of total flavonoids from peppermint by polyamide. Methods: The total flavonoids content and adsorption - elution rate as an indicator, using single factor experiments to examine the adsorption capacity of five different types of purified materials on total flavonoids, to determine the purified material, and its purification process adsorption conditions, impurity removal conditions And elution conditions to optimize. Results: The optimum conditions were as follows: the mass concentration of the sample was 0.1 g.mL-1, the sample volume was 1: 6, the flow rate was 2 BV.h-1, After the adsorption was finished, the mixture was eluted with 3 BV 10% ethanol, the impurity removal rate was 4 BV.h-1, and then eluted with 3 BV 90% ethanol at a flow rate of 8 BV.h-1. The polyamide ~ 60 mesh) purified, mint flavonoids purity up to 50%. Conclusion: The optimized purification process has the advantages of good separation effect, simple operation and good repeatability, and can be used for separation and enrichment of mint flavonoids.