目的:研究星形胶质细胞升高基因1(AEG-1)调控细胞自噬及上皮间质转化(EMT)诱导甲状腺乳头状癌(PTC)增殖和转移及其作用机制。方法:体外培养正常甲状腺细胞和PTC细胞。实时荧光定量PCR(qRT-PCR)检测AEG-1在PTC细胞中的表达，选择AEG-1最高表达的PTC细胞进行AEG-1 shRNA的感染，分为sh-NC组和sh-AEG-1组。采用CCK8和EdU(5-乙炔基-2′-脱氧尿苷)实验检测AEG-1对PTC细胞增殖能力的影响；Transwell小室实验检测AEG-1对PTC细胞转移能力的影响；裸鼠皮下成瘤实验检测AEG-1对PTC细胞体内生长能力的影响；Western blot检测AEG-1对自噬相关蛋白和EMT相关蛋白表达影响。分别采用自噬诱导剂Rapamycin和EMT诱导剂转化生长因子-β(TGF-β)处理sh-NC组和sh-AEG-1组PTC细胞，采用CCK8和Transwell小室实验分别检测各组细胞的增殖和转移能力。结果:与正常甲状腺细胞Nthy-ori3-1相比，PTC细胞中AEG-1的表达水平增加，其中在TPC-1细胞中表达最高。AEG-1 shRNA转染TPC-1细胞后，细胞的增殖、转移和体内成瘤能力均降低(n P<0.05)。与sh-NC组相比，sh-AEG-1组细胞中自噬相关蛋白P62、Beclin1表达增加和LC3B蛋白表达降低，EMT相关蛋白E-cadherin表达增加和N-cadherin、Vimentin蛋白表达降低(n P<0.05)。CCK8和Transwell小室实验表明自噬诱导剂Rapamycin和EMT诱导剂TGF-β处理均减弱了sh-AEG-1对PTC细胞增殖和转移能力的抑制作用(n P<0.05)。n 结论:AEG-1通过诱导细胞自噬及EMT促进PTC细胞增殖和转移能力。“,”Objective:To study the effect of astrocyte elevated gene-1 (AEG-1) on proliferation and metastasis of papillary thyroid cancer (PTC) by regulating cell autophagy and epithelial-mesenchymal transition (EMT).Methods:Normal thyroid cells Nthy-ori3-1 and PTC cells TPC-1, FTC-133, B-CPAP and SW579 were cultured in n vitro. Real time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of AEG-1 in PTC cells. The PTC cells with the highest AEG-1 expression were selected for AEG-1 shRNA infection, and then divided into sh-NC group and sh-AEG-1 group. Cell counting kit-8 (CCK8) and EdU (5-ethynyl-2 ′- deoxyuridine) experiments were used to detect the effect of AEG-1 on the proliferation of PTC cells; Transwell test was used to detect the effect of AEG-1 on the metastasis of PTC cells; subcutaneous tumorigenesis test in nude mice was used to detect the effect of AEG-1 on the expression of autophagy related proteins and EMT related proteins. PTC cells in sh-NC group and sh-AEG-1 group were treated with rapamycin and transforming growth factor-β (TGF-β), respectively. CCK8 and transwell assay were used to detect the cell proliferation and metastasis ability of each group of cells, respectively.n Results:Compared with normal thyroid cells Nthy-ori3-1, the expression level of AEG-1 in PTC cells was increased, with the highest expression in TPC-1 cells. After AEG-1 shRNA was transfected into TPC-1 cells, cell proliferation, metastasis and tumorigenicity in n vivo were reduced (n P<0.05). Compared with the sh-NC group, the expression of autophagy-related proteins P62 and Beclin1 were increased and the expression of LC3B protein was decreased, and EMT-related proteins E-cadherin expression were increased and N-cadherin and Vimentin protein expression were decreased in the sh-AEG-1 group (n P<0.05). CCK8 and Transwell experiments showed that treatment with autophagy inducer Rapamycin and EMT inducer TGF-β attenuated the inhibitory effect of sh-AEG-1 on proliferation and metastasis ability of PTC cell (n P<0.05).n Conclusions:AEG-1 promotes the proliferation and metastasis of PTC cells by inducing cell autophagy and EMT.