Retroviral mediated gene transfer of humanglobin gene into hematopoietic stem cells is apromising approach for thalassemia gene therapy.Major problem of the transferred globin gene was lowlevel expression of the gene with its proximal cis-acting sequence. The locus control region (LCR) ofthe human β-globin gene cluster consists of four majorDNase I hypersensitive sites (HS). When linked to Retroviral mediated gene transfer of humanglobin gene into hematopoietic stem cells is apromising approach for thalassemia gene therapy. Major problem of the transmitted globin gene was lowlevel expression of the gene with its proximal cis-acting sequence. The locus control region (LCR) of the human β -globin gene cluster consists of four majorDNase I hypersensitive sites (HS). When linked to