乌头碱是乌头属植物中所含的二萜双酯型生物碱类中的一种,成人口服纯品致死量仅约5mg,由乌头属植物引起的中毒与死亡屡见报导但一直缺乏较好的检测方法,使中毒后体内乌头碱分布与分解规律的研究难以进行,也给这类中毒案件的法医学鉴定带来一定困难。近年来应用HPLC分离检测生药中乌头碱类生物碱的方法已有报导。本文应用HPLC Aconitine is one of the diterpene diester-type alkaloids contained in the genus Aconitum, which is commonly reported to be only about 5 mg in the oral dose of adult oral administration, and poisoning and death caused by the genus Aconitum have been reported for a long time The lack of better detection methods makes it difficult to study the distribution and decomposition of aconitine in vivo after poisoning, and brings some difficulties to forensic identification of such poisoned cases. In recent years, the application of HPLC separation of aconitum alkaloids in crude drugs has been reported. This application of HPLC