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介绍了一种用于对肝磷脂中过硫酸化软骨素硫酸盐(OSCS)分离和定量的高效液相色谱(HPLC)方法。该方法使用阴离子交换色谱实现了肝磷脂和OSCS的完全分离,并用紫外吸收法对肝磷脂和OSCS进行了定量。实验结果表明,该方法不但具有重现性好和分析速度快(10min)的优点,而且可以在OSCS浓度低于总浓度1%时也能够对其进行有效检测。这种HPLC方法突破了毛细管电泳分析肝磷脂方法固有的局限性。
A high performance liquid chromatography (HPLC) method for the isolation and quantification of the over-sulfated chondroitin sulfate (OSCS) in heparin is described. This method uses anion exchange chromatography to achieve a complete separation of heparin and OSCS, and heparin and OSCS are quantified by UV absorption. The experimental results show that this method not only has the advantages of good reproducibility and fast analysis speed (10min), but also can effectively detect the OSCS when its concentration is less than 1% of the total concentration. This HPLC method breaks the inherent limitations of the capillary electrophoresis assay of heparin.