Breeding of Maintainer Line and Sterile Line Brown Planthopper Resistant Rice Using Marker-assisted

来源 :Asian Agricultural Research | 被引量 : 0次 | 上传用户:sunday_rectina
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Guangxi common wild rice variety brown planthopper highly resistant introgression line HS204 was taken as antigen donor material,hybridization,backcrossing,and molecular marker-assisted selection methods were adopted,to select maintainer line and sterile line materials,so as to provide excellent material foundation for resistance breeding of hybrid rice. Through the marker-assisted selection,it obtained 4 pieces of homozygous resistance gene maintainer line intermediate materials( 100B,101B,102B and 103B),and 2 pieces of resistant sterile line materials( 100A and 103A). All 10 combinations that have testcross with highly resistant sterile 100A showed higher level of brown planthopper resistance: 100A/R2586,100A/KR838,and 100A/KR527 had high resistance level,the others had low to intermediate resistance;100A/KR527,100A/R2586,100A/Minghui 63,100A/Fuhui 838 and 100A/Gui 99 combinations had yield per plant significantly higher than the control group( Teyou 7118),increasing by 14. 45%-49. 26%. The obtained resistant lines are expected to provide a better gene source for the breeding of resistant sterile lines of hybrid rice and the obtained resistant sterile lines can be directly used in the selection of three-line hybrid rice. Guangxi common wild rice variety brown planthopper highly resistant introgression line HS204 was taken as antigen donor material, hybridization, backcrossing, and molecular marker-assisted selection methods were adopted, to select maintainer line and sterile line materials, so as to provide excellent material foundation for resistance breeding of hybrid rice. Through the marker-assisted selection, it was obtained 4 pieces of homozygous resistance gene maintainer line intermediate materials (100B, 101B, 102B and 103B), and 2 pieces of resistant sterile line materials (100A and 103A) 10 combinations that have highly resistant sterile 100A showed higher level of brown planthopper resistance: 100A / R2586, 100A / KR838, and 100A / KR527 had high resistance level, the others had low to intermediate resistance; 100A / KR527, 100A / R2586 , 100A / Minghui 63,100A / Fuhui 838 and 100A / Gui 99 combinations had yield per plant significantly higher than the control group (Teyou 7118), increasing by 14. 45% -49. 2 6%. The acquired resistant lines are expected to provide a better gene source for the breeding of resistant sterile lines of hybrid rice and the acquired resistant sterile lines can be directly used in the selection of three-line hybrid rice.
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