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大鼠乙醚麻醉后,分别在左右后脚掌上粘上盘形记录电极各1个,同时经右后肢膝韧带向膝关节内注入30％尿酸矿油混悬液0.05ml。这种方法可引起炎症、疼痛和运动功能损害,注射后2.5h最重,持续6.5 h。本文采用转筒法测定关节损伤后大鼠的行走能力来评估药物的镇痛作用。水平转筒由长60 cm,直径30 cm的中空不锈钢圆筒和外包的防滑金属网组成。筒身分隔成6道,故可同时观察6只动物。转筒由马达带动,保持4转/min的速度以迫使大鼠行走,正常鼠只需1 min即可
Rats anesthetized with ether, respectively, the left and right posterior palms sticky disk-shaped recording electrode 1, while the right hind knee ligament into the knee joint injection of 30% uric acid mineral oil suspension 0.05ml. This method can cause inflammation, pain and motor function damage, 2.5h after injection the heaviest, sustained 6.5 h. In this paper, the determination of the ability of the rats to walk after the joint injury by walking to assess the analgesic effect of the drug. The horizontal drum consists of a hollow stainless steel cylinder 60 cm long and 30 cm in diameter with a non-slip metal mesh. Body separated into 6, it can be observed at the same time 6 animals. Drum driven by the motor to maintain the speed of 4 rev / min to force rats to walk, normal rats only 1 min