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以耐盐性不同的紫花苜蓿(Medicago sativa L.)品种“阿尔冈金”和“皇冠”为试材,在水培条件下,研究了不同盐胁迫浓度(0、100、200mmol/L NaCl浓度)和不同胁迫时间(0、2、4、6、8、10、12d)对2个紫花苜蓿品种幼苗的根系活力、丙二醛含量、脯氨酸含量、可溶性蛋白质含量等相关根部生长及生理指标的影响。结果表明:随着盐胁迫浓度和天数的增加,紫花苜蓿根系活力呈现明显下降的趋势;随着盐胁迫浓度和天数的增加,紫花苜蓿根部脯氨酸含量、丙二醛含量和可溶性性蛋白质含量均呈现明显的先升高后降低的趋势,但是高盐溶液升高的趋势要明显大于低盐溶液升高的趋势。经主成分分析表明,脯氨酸含量和可溶性蛋白质含量能较好的代表紫花苜蓿的耐盐性。
The effects of different salt stress concentrations (0, 100, 200 mmol / L) on the growth of alfalfa (Medicago sativa L.) varieties “Algonquin” and “Crown” / L NaCl concentration) and different stress time (0, 2, 4, 6, 8, 10 and 12 d) on the root activity, malondialdehyde content, proline content and soluble protein content of the two alfalfa varieties Root growth and physiological indicators. The results showed that with the increase of salt stress concentration and days, the root activity of alfalfa decreased obviously. With the increase of salt stress concentration and days, the content of proline, malondialdehyde and soluble protein in alfalfa roots All showed a clear trend of first increasing and then decreasing. However, the tendency of high salt solution to increase was obviously greater than that of low salt solution. The principal component analysis showed that proline content and soluble protein content can better represent the salt tolerance of alfalfa.